The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. TMB is then used to visualize HRP enzymatic reaction. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. NOT FOR USE IN DIAGNOSTIC PROCEDURES.Corticosterone ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.Ī Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Please read this insert completely prior to using the product FOR RESEARCH USE ONLY.
#DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT SOFTWARE#
The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The substrate reacts with the bound corticosterone-peroxidase conjugate.
After an hour incubation the plate is washed and substrate is added. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies.
Please choose the standard range that fits your sample concentrations most appropriately. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. This kit measures total corticosterone in serum and plasma and in extracted fecal samples.Ī corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please read the complete kit insert before performing this assay. The DetectX® Corticosterone Immunoassay kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. You need JavaScript enabled to view it.Ĭorticosterone (C 21H 30O 4, Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. This email address is being protected from spambots. You need JavaScript enabled to view it." style=""> This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica line-height: 15.59375px "> You need JavaScript enabled to view it." style="font-size: 12px line-height: 1.3em ">įax (+49) 0241 56 00 47 88 This email address is being protected from spambots.
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